| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tolan, Sydney T (10)
|
ADSSCY |
48.85 |
46.38 |
-2.47 |
| 2 |
Manion, Anna R (9)
|
ADWAVE |
55.68 |
52.57 |
-3.11 |
| 3 |
Marcotullio, Caitlyn A (10)
|
ADADDI |
54.90 |
53.25 |
-1.65 |
| 4 |
Sliper, Kaylee P (10)
|
ADBSPA |
54.59 |
53.50 |
-1.09 |
| 5 |
Kabeli, Sophie A (10)
|
ADWAVE |
57.71 |
54.05 |
-3.66 |
| 6 |
Lutz, Olivia G (10)
|
ADJJSC |
|
57.42 |
- |
| 7 |
Ahl, Grace E (9)
|
ADBSPA |
1:01.83 |
58.99 |
-2.84 |
| 8 |
Lawrence, Renee E (9)
|
ADJJSC |
1:03.52 |
1:01.08 |
-2.44 |
| 9 |
McCord, Abby E (10)
|
ADKING |
59.39 |
1:02.15 |
2.76 |
| 10 |
Katz, Kaya V (9)
|
ADBSPA |
|
1:15.60 |
- |
| 11 |
Parker, Elise H (10)
|
ADADDI |
|
1:21.54 |
- |
| 12 |
Eliea-Shields, Madelyn R (9)
|
ADDELM |
|
1:46.06 |
- |
|
Brandl, Kristin W (9)
|
ADBSPA |
1:24.03 |
DQ |
- |
|
Dolan, Molly Z (9)
|
ADBSPA |
|
DQ |
- |
|
Lundin, Paige R (9)
|
ADBSPA |
|
DQ |
- |
|
Manion, Nora E (9)
|
ADBSPA |
|
DQ |
- |